TCID50 Assay
Plaque assay and TCID50 assay are helpful methods for determining viral titers. TCID50 assays are being used to determine the infectious titer of any such virus types, which can cause cytopathic effects (CPE, structural changes in a host cell resulting from viral infection) in tissue culture over a reasonable period of 5 to 20 days while cells in culture remain viable.
TCID50 (50% tissue culture infectious doses) signifies the concentration at which 50% of the cells are infected when a test tube or well plate upon which cultured cells have been inoculated with a diluted solution of viral fluid. The TCID50 assay is one of the methods for determining the titration of a virus. The TCID50 assay is used to quantify viral titers by determining the concentration at which 50% of the infected cells display cytopathic effect. This method relies on the morphological change of the cell when it has been infected by the virus. The procedure is performed to determine the infectious titer of any virus that can cause cytopathic effects in tissue culture and is carried out for 5 to 20 days in culture to remain viable. CPE occurs when the infecting virus causes lysis (dissolution) of the host cell or when the cell dies without lysis because of its inability to reproduce. The degree of CPE can indirectly reflect the virulence of the virus, so this feature can be used to determine the virulence of the virus. It should be noted that not all viruses can cause CPE in tissue culture. Cell lines and viruses should be aligned to see a cytopathic effect.
Creative Diagnostics provides TCID50 Assay Service with a high level of assay sensitivity and accuracy. We have rich experience serving pharmaceutical, biotechnology, contract research, and academic scientists. Our TCID50 assays are used to assay drug sensitivity, quantify infectious units, determine the dose of virus required and study virus release.
Our Procedures
Our Features
- It is cheap and easy to implement also when virus-specific antibodies are not available.
- Very little information on the virus itself is required.
- Compared with plaque assay method, the error is small.
- High sensitivity and accuracy.
Our Capabilities
- Cytomegalovirus (CMV)
- Dengue virus (DENV)
- Hepatitis B virus (HBV)
- Hepatitis C virus (HCV)
- Herpes simplex virus (HSV)
- Human cytomegalovirus (HCMV)
- Human immunodeficiency virus (HIV)
- Human rhinovirus (HRV)
- Influenza virus (IFV)
- Respiratory syncytial virus
- Ross River virus (RRV)
- Semliki forest virus (SFV)
- Sindbis virus (SINV)
- Vaccinia virus (VACV)
- Zika virus (ZIKA)
(Other viruses or microorganisms may be available on request)
We combine infection and analytical expertise to provide our clients with the most powerful portfolio of antiviral and antimicrobial in vitro testing services. Facing an increasing demand for new antiviral and antimicrobial compounds for the treatment of infectious diseases, Creative Diagnostics can test these compounds in vitro to determine their potential efficacy in vivo models.
