Colon Ascendens Stent Peritonitis (CASP) Model
The ascending colon stent peritonitis (CASP) model was reported for the first time in 1997, which is a model of endogenous fecal contamination. CASP represents a highly standardized rodent polymicrobial abdominal sepsis model, which firstly leads to diffuse peritonitis, and secondly, an early and stable increase in systemic infection and inflammation.
The pathogenesis of sepsis is very complicated. The main pathophysiological processes include cytokine storm, inflammatory waterfall-like reaction, enteral bacterial translocation, intestinal-derived endotoxin, the interplay between coagulation and inflammation systems, oxidative stress, immune cell apoptosis, microcirculation, and mitochondrial dysfunction. Ideal animal models of sepsis have been widely used, such as endotoxemia models, cecal ligation and puncture (CLP) models, and CASP models. CLP and CASP models can better simulate the pathophysiological process of human sepsis and are currently the most popular models.
In the CASP model, a small stent is surgically inserted into the ascending colon of a mouse or rat, causing intestinal bacteria to continuously infiltrate the peritoneal cavity. This process can lead to peritonitis, systemic bacteremia, infection of organs by intestinal bacteria, and the systemic and local release of several pro-inflammatory and anti-inflammatory cytokines. The lethality of CASP can be controlled by the diameter of the stent. CASP is a simple, easy-to-learn, reproducible model of abdominal sepsis, which can well simulate the clinical course of abdominal sepsis. It guides people to study issues in several scientific fields, such as immunology, infection, and surgery.
Creative Diagnostics believes that animal models are very useful for studying human diseases because in vitro studies cannot reflect the full range of anatomical and physiological effects. The use of laboratory animals in biomedical research is a key factor in the development of new prevention methods and the treatment of infectious and non-communicable diseases. Our experienced professional team can provide standard in vivo prediction models of peritonitis for drug discovery and treatment efficacy studies that need to be developed and validated.
Our Procedures
- Preparation of the animal
- Operation
- Postoperative care
- CASP
- Analysis
Our Services
- CASP model development
- Types: CASP & CASP with intervention.
- Methods: various needle sizes, number of punctures and length of ligation.
- Animal species: rat, mouse, etc.
- Behavioral monitoring
- Appearance of eyes (open, closed, secretions)
- Appearance of fur (piloerection, soiling, etc.)
- Bleeding from an orifice
- Evaluation of respiratory rate and associated noise/gasping
- Posture
- Presence or absence of activity (foraging, eating, etc.) and assessment of ataxia
- Quality of bowel movements
- Response to stimulation
- Self-mutilating behavior
- Weight loss, level of food/water intake
Our Advantages
- Custom animal model development
- Comprehensive evaluation of drug candidates
- Experienced staffs for performing the operation exactly and quickly
- Full report and timely result delivery
Creative Diagnostics is a full-service contract research organization specializing in microbiology and preclinical infectious disease research. The correct animal model provides unlimited possibilities for drug discovery and human health advancement. At Creative Diagnostics, we believe that providing clients with the best animal model solutions is the most effective way to promote key research from creativity to reality. We guarantee that the animal models required in research are strictly regulated to ensure responsible, ethical, and humane treatment.
