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Fluorometric Neuraminidase Assay

Neuraminidase (NA), also known as sialidase, is a glycoprotein distributed on the envelope of the influenza virus. It has antigenicity and can catalyze sialic acid hydrolysis to help mature the influenza virus break away from host cells and infect new cells. It plays an essential role in the life cycle of the influenza virus. The most common neuraminidase is viral neuraminidase. The binding and division of sialic acid and adjacent sugar groups allow the virus to transport through mucin, destroying the host cell's hemagglutinin receptor, thus allowing the release of offspring virions from infected cells. Neuraminidase promotes the spread of respiratory viruses by stimulating the release of influenza viruses from infected cells. Therefore, it is an essential target for the development of influenza drugs. The detection of neuraminidase and neuraminidase inhibitors (NI) via fluorometric neuraminidase assay are the primary tasks to study the biological process and prevent influenza infection.

Creative Diagnostics measures a fluorogenic substance released by the active influenza neuraminidase enzyme. We will titer the viral isolates based upon neuraminidase activity. Subsequently, neuraminidase enzyme inhibition assays and antiviral drug activity screening will be conducted to determine the 50% drug inhibitory dose.

Our Procedures

Fluorometric Neuraminidase Assay

Our Features

  • We provide a simple and flexible format for screening several to hundreds of virus isolates or screening thousands of compounds with high confidence and high-quality data during high-throughput lead discovery.
  • Fluorometric neuraminidase assay is suitable for the functional detection of NI-resistant virus in a mixed virus population.
  • Fluorometric neuraminidase assay can also be used to monitor the neuraminidase activity of non-viral or bacterial origin.

Our Capabilities

  • Influenza A (H1N1) virus
  • Influenza A (H3N2) virus
  • Influenza B (Victoria-lineage) virus
  • Influenza B (Yamagata-lineage) virus
  • H9N2, H7N9, and attenuated H5N1 (BSL-2)

(Other viruses or microorganisms may be available on request)

We combine infection and analytical expertise to provide our clients with the most powerful portfolio of antiviral and antimicrobial in vitro testing services. Facing an increasing demand for new antiviral and antimicrobial compounds for the treatment of infectious diseases, Creative Diagnostics can test these compounds in vitro to determine their potential efficacy in vivo models.

Services

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We Are Providing Our Clients Antiviral & Antibacterial Services

Having extensive knowledge in cell biology and the application of cell-based assays to benefit clients in the drug and vaccine development industry

Offering comprehensive solutions to drive your projects to completion

Delivering prompt quotations and competitive prices

Providing quality science and personalized customer service

Antiviral Services - Creative Diagnostics

Creative Diagnostics provides expertise in antiviral (or antibacterial) assays and cell-based antiviral (or antibacterial) compound screening to other biotechnology and pharmaceutical companies.

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