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Human Papillomavirus (HPV) Neutralization Assay

Cervical cancer is one of the most common cancers in women worldwide. It is reported that the vast majority (more than 95%) of cervical cancers are caused by human papillomavirus (HPV). Reproductive tract viral infection is the most common route. Currently, four vaccines have been prequalified to provide protection against HPV types 16 and 18. These two types of HPVs cause at least 70 percent of cervical cancers. The 9-valent HPV vaccine protects against five other carcinogenic human papillomaviruses, which account for 20% of cervical cancer cases. Two of the vaccines also provide protection against human papillomavirus types 6 and 11, which cause anogenital warts.

HPV-neutralization-assayFigure 1. HPV classification.

Besides the current vaccines, people keep developing new generation HPV vaccines and drugs. HPV neutralization assay is the essential step for the pre-clinic screening. The most authoritative methodology of evaluating anti-HPV activity is pseudovirius-based neutralization assay (PBNA).

Creative Diagnostics provide the pseudotyped GFP HPV which is generated on 293FT cell line and stalely expressed codon-modified papillomavirus capsid genes, L1 and L2. With the GFP signal of each pseudovirions, it is easy to process mid-scale and large-scale screening test of anti-HPV candidates. In general, we perform the neutralization assay on VERO cell line and readout the fluorescence signal of GFP. We also offer the antibody staining-based neutralization for certain serotypes. Please contact us for the further information.

Table 1. HPV Neutralization Assay list

Serotype Reporter
HPV-6 GFP
HPV-11 GFP
HPV-16 GFP
HPV-18 GFP
HPV-31 GFP
HPV-33 GFP
HPV-45 GFP
HPV-52 GFP
HPV-58 GFP

HPV-neutralization-assayFigure 2. HPV neutralization assay.

Why Use Our HPV Antibody Testing Services?

  • Two assays available to measure 9-valent HPV neutralizing antibodies qualitatively and quantitatively
  • Superior quality and competitive pricing
  • We accept samples from anywhere in the world every day
  • May be able to transfer client's custom assays in-house to perform routine screening
  • ELISA assays available to detect antibody against different HPV serotypes in serum

Frequently Asked Questions (FAQs) About HPV Neutralizing Antibody Testing

  • What types of AAV Neutralization assays do you offer?

We offer a NAb screening assay (+ or – at dilution of 1:10, 1:20, and 1:40) which is a qualitative assay at various dilutions to detect the presence of pre-existing neutralizing antibodies. We also offer a NAb titer assay, which is a quantitative assay to measure EC50 of antibody in serum.

  • What regulatory levels do you offer for HPV Antibody Neutralization Assays?

We offer an RUO (research use only) service to detect neutralization activity.

Quotations and Ordering

Quotations-and-Ordering

Reference:

  1. Wang, X., Huang, X., & Zhang, Y. (2018). Involvement of human papillomaviruses in cervical cancer. Frontiers in microbiology, 9, 2896.

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We Are Providing Our Clients Antiviral & Antibacterial Services

Having extensive knowledge in cell biology and the application of cell-based assays to benefit clients in the drug and vaccine development industry

Offering comprehensive solutions to drive your projects to completion

Delivering prompt quotations and competitive prices

Providing quality science and personalized customer service

Antiviral Services - Creative Diagnostics

Creative Diagnostics provides expertise in antiviral (or antibacterial) assays and cell-based antiviral (or antibacterial) compound screening to other biotechnology and pharmaceutical companies.

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