Minimum Doubling Time/Growth Curve (MDT)
Under favorable conditions, microorganisms constantly absorb nutrients and carry out metabolic activities in accordance with their own metabolic patterns. If the assimilation effect is greater than the alienation effect, the amount of microbial cytoplasm will continue to increase as well as its volume, so the microorganism is in the growth.
The growth of single-celled microorganisms such as bacteria is often accompanied by an increase in the number of cells. When the cell grows to a certain extent, it forms two basically identical daughter cells in the form of binary fission, and the daughter cells will repeat the above process. In single-celled microorganisms, the increase in the number of individuals caused by cell division is called reproduction. In general, when the environmental conditions are suitable, growth and reproduction are always carried out alternately. From growth to reproduction is a process from qualitative change to quantitative change, and this process is development.
If physical and chemical conditions suit, microorganisms can grow and develop normally, and the reproduction rate is also high; if one or some environmental conditions change and go beyond the range that organisms can adapt to, it will have an inhibitory or even killing effect on the microorganisms. Therefore, by measuring the growth curve of microorganisms, we can understand the growth law of all kinds of bacteria and microorganisms, which has important guiding significance for scientific research and production.
Moreover, the minimum doubling time (MDT) test is used to determine the time it takes for the number of bacteria to double. This experiment illustrates the effect of antibiotics on bacterial growth by comparing the effects of adding and not adding antibacterial compounds on the growth of bacteria in broth culture.
There are many different methods for determining the number of microorganisms, which can be selected according to the requirements and laboratory conditions. Because the concentration of bacterial suspension is proportional to the optical density (OD), Creative Diagnostics generally uses a spectrophotometer to evaluate the OD of bacterial suspension to deduce the concentration of bacterial suspension, so as to draw the growth curve and calculate minimum doubling time.
Our Procedures
Our Features
- Tailored to your project.
- Record ODs at multiple timepoints.
- Use ODs recorded in the exponential phase of growth to calculate minimum doubling time.
- Various measurements available to obtain data for calculating.
Our Capabilities
- Acinetobacter baumannii
- Bacillus subtilis
- Enterobacter aerogenes
- Enterobacter cloacae
- Escherichia Coli
- Methicillin-Resistant Staphylococcus aureus
- Methicillin-Sensitive Staphylococcus aureus
- Moraxella catarrhalis
- Pseudomonas aeruginosa
- Streptococcus pneumoniae
(Other microorganisms may be available on request)
We combine infection and analytical expertise to provide our clients with the most powerful portfolio of antiviral and antimicrobial in vitro testing services. Facing an increasing demand for new antiviral and antimicrobial compounds for the treatment of infectious diseases, Creative Diagnostics can test these compounds in vitro to determine their potential efficacy in vivo models.
